Vázquez Bigas, GuillemEspargaró Colomé, AlbaCaballero Hernández, Ana BelénDi Pede Mattatelli, AniaBusquets i Viñas, Ma. AntoniaNawrot, DariaSabaté Lagunas, RaimonNicolás Galindo, ErnestoJuárez Jiménez, JordiGámez Enamorado, Patrick2025-09-262025-09-262024-12-010143-7208https://hdl.handle.net/2445/223397A modified lysine residue containing an environment-sensitive moiety was prepared through a straightforward synthesis, and its fluorescent properties were examined. The new fluorescent sensor, DMN-BocK, can monitor amyloid aggregation processes associated with neurodegenerative diseases such as Alzheimer’s or Parkinson’s. DMN-BocK offers advantages over classical amyloid-specific dyes like Thioflavins or Congo Red because it is (1) available to detect a broader range of amyloid structures; (2) useful both in vitro and in cellulo; (3) capable of differentiating amyloid structures, providing information on the binding site microenvironment; and (4) a synthon than can be incorporated into protein sequences to gain further structural information. Our findings suggest that DMN-based amino-acid probes have a strong potential to become a sensor of choice for in vitro and in cellulo studies of amyloid aggregation in drug discovery assays.10 p.application/pdfengcc-by-nc (c) Vázquez Bigas, Guillem et al., 2024http://creativecommons.org/licenses/by-nc/3.0/es/ProteïnesMalalties neurodegenerativesAmiloidesEspectroscòpia de fluorescènciaProteinsNeurodegenerative DiseasesAmyloidFluorescence spectroscopyinfo:eu-repo/semantics/article7499012025-09-26info:eu-repo/semantics/openAccess