Fernández, AlejandroGairí Tahull, MargaridaGonzález, Maria TeresaPons Vallès, Miquel2025-07-222025-07-222024-11-080022-2623https://hdl.handle.net/2445/222479Methionine residues within the kinase domain of Src serve as unique NMR probes capable of distinguishing between distinct conformational states of full-length Src, including alternative drug-inhibited forms. This approach offers a rapid method to differentiate between various inhibition mechanisms at any stage of drug development, eliminating the need to resolve the structure of Src-drug complexes. Using selectively 13C-methyl-enriched methionine, spectra can be acquired in under an hour, while natural abundance spectra with comparable information are achievable within a few hours.9 p.application/pdfengcc-by (c) Fernández, Alejandro et al., 2024http://creativecommons.org/licenses/by/3.0/es/Biologia molecularMonòmersPèptidsMolecular biologyMonomersPeptidesinfo:eu-repo/semantics/article7576632025-07-22info:eu-repo/semantics/openAccess