Mannara, F.Valente, TonySaura Martí, JosepGraus Ribas, FrancescSaiz Hinarejos, AlbertMoreno, Beatriz2014-04-282014-04-282012-12-261932-6203https://hdl.handle.net/2445/53665Experimental autoimmune encephalomyelitis (EAE) is the most relevant animal model to study demyelinating diseases such as multiple sclerosis. EAE can be induced by active (active EAE) or passive (at-EAE) transfer of activated T cells in several species and strains of rodents. However, histological features of at-EAE model in C57BL/6 are poorly described. The aim of this study was to characterize the neuroinflammatory and neurodegenerative responses of at-EAE in C57BL/6 mice by histological techniques and compare them with that observed in the active EAE model. To develop the at-EAE, splenocytes from active EAE female mice were harvested and cultured in presence of MOG 35-55 and IL-12, and then injected intraperitoneally in recipient female C57BL6/J mice. In both models, the development of EAE was similar except for starting before the onset of symptoms and presenting a higher EAE cumulative score in the at-EAE model. Spinal cord histological examination revealed an increased glial activation as well as more extensive demyelinating areas in the at-EAE than in the active EAE model. Although inflammatory infiltrates composed by macrophages and T lymphocytes were found in the spinal cord and brain of both models, B lymphocytes were significantly increased in the at-EAE model. The co-localization of these B cells with IgG and their predominant distribution in areas of demyelination would suggest that IgG-secreting B cells are involved in the neurodegenerative processes associated with at-EAE.9 p.application/pdfengcc-by (c) Mannara et al., 2012http://creativecommons.org/licenses/by/3.0/esMalalties neurodegenerativesCèl·lules BEncefalomielitisNeurodegenerative DiseasesB cellsEncephalomyelitisinfo:eu-repo/semantics/article6236202014-04-28info:eu-repo/semantics/openAccess23300649