Rodríguez Allué, Manuel JoséGuerras Garcia, Àngels2023-06-202023-06-202023-06-07https://hdl.handle.net/2445/199506Treballs Finals de Grau d'Enginyeria Biomèdica. Facultat de Medicina i Ciències de la Salut. Universitat de Barcelona. Curs: 2022-2023. Tutor/Director: Rodríguez Allué, Manuel JoséNeurodegenerative movement-related disorders are characterized by a progressive degeneration and loss of neurons, which lead to motor control impairment. Although the precise mechanisms underlying these conditions are still unknown, an increasing number of studies point towards the analysis of neural networks and functional connectivity to unravel novel insights. The main objective of this work is to understand cellular mechanisms related to dysregulated motor control symptoms in movement disorders, such as Chorea-Acanthocytosis (ChAc), by employing multielectrode arrays to analyze the electrical activity of neuronal networks in mouse models. We found no notable differences in cell viability between neurons with and without VPS13A knockdown, that is the only gene known to be implicated in the disease, suggesting that the absence of VPS13A in neurons may be partially compensated by other proteins. The MEA setup used to capture the electrical activity from neuron primary cultures is described in detail, pointing out its specific characteristics. At last, we present the alternative backup approach implemented to overcome the challenges faced during the research process and to explore the advanced algorithms for signal processing and analysis. In this report, we present a thorough account of the conception and implementation of our research, outlining the multiple limitations that have been encountered all along the course of the project. We provide a detailed analysis on the project’s economical and technical feasibility, as well as a comprehensive overview of the ethical and legal aspects considered during the execution.70 p.application/pdfengcc-by-nc-nd (c) Guerras Garcia, Àngels, 2023http://creativecommons.org/licenses/by-nc-nd/3.0/es/Enginyeria biomèdicaCorea de SydenhamTreballs de fi de grauCorea de HuntingtonTranstorns motorsProcessament de senyalsXarxes neuronals (Neurobiologia)ElectrofisiologiaBiomedical engineeringChoreaBachelor's thesesHuntington's choreaMovement disordersSignal processingNeural networks (Neurobiology)Electrophysiologyinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/openAccess