Teixidó Condomines, ElisabetPiqué Benages, Maria EstherGómez Catalán, JesúsLlobet Mallafré, Joan M. (Joan Maria)2020-06-122020-06-122013-02-270887-2333https://hdl.handle.net/2445/165289In this study we analyzed some aspects of the assessment of developmental delay in the zebrafish embryotoxicity/teratogenicity test and explored the suitability of acetylcholinesterase (AChE) activity as a biochemical marker and as a higher throughput alternative to morphological endpoints such as head-trunk angle, tail length and morphological score. Embryos were exposed from 4 to 52 h post-fertilization (hpf) to a selection of known embryotoxic/teratogen compounds (valproic acid, retinoic acid, caffeine, sodium salicylate, glucose, hydroxyurea, methoxyacetic acid, boric acid and paraoxon-methyl) over a concentration range. They were evaluated for AChE activity, head-trunk angle, tail length and several qualitative parameters integrated in a morphological score. In general, the different patterns of the concentration-response curves allowed distinguishing between chemicals that produced growth retardation (valproic and methoxyacetic acid) and chemicals that produced non-growth-delay related malformations. An acceptable correlation between the morphological score, AChE activity and head-trunk angle as markers of developmental delay was observed, being AChE activity particularly sensitive to detect delay in the absence of malformations.10 p.application/pdfeng(c) Elsevier Ltd, 2013EnzimologiaEmbriologiaMetabolismeAcetilcolinesterasaNanismeMalformacionsPeix zebraToxicologiaEnzymologyEmbryologyMetabolismAcetylcholinesteraseDwarfismHuman abnormalitiesZebra danioToxicologyinfo:eu-repo/semantics/article6159872020-06-12info:eu-repo/semantics/openAccess