Pino Saladrigues, Marta delMartí Delgado, CristinaGaber, JinaSvanholm-Barrie, CeciliaRodríguez-Carunchio, LeonardoRodríguez Trujillo, AdrianoCarreras-Dieguez, NuriaFuertes, IreneBarnadas, EstherMarimon, LorenaBlanco, José L.Persing, David H.Torné Bladé, AureliOrdi i Majà, Jaume2020-06-112020-06-112019-11-022075-4418https://hdl.handle.net/2445/165174There is growing interest in anal cancer screening strategies. However, cytological/molecular evaluation of anal samples is challenging. We aimed to determine the feasibility of detecting, in anal liquid-based cytologies, the expression of biomarkers involved in the cell cycle disturbance elicited by human papillomavirus (HPV). The accuracy of this approach in the identification of high-grade squamous intraepithelial lesions/anal intraepithelial neoplasia grade2-3 (HSIL/AIN2-3) was also evaluated. 215 anal cytologies from men having sex with men living with human immunodeficiency virus were evaluated. Patients showing concordant cytological and anoscopy-directed biopsy diagnosis were selected: 70 with negative cytology and HPV test, 70 with low-grade SIL (LSIL/AIN1) cytology and biopsy, and 75 with cytology and biopsy of HSIL/AIN2-3. CDKN2A/p16, MKI67 and TOP2A mRNA expression was analyzed. HPV detection was performed with Xpert HPV Assay (Cepheid, Sunnyvale, CA, USA). HSIL/AIN2-3 showed higher expression for the biomarkers than LSIL/AIN1 or negative samples. The specificity for HSIL/AIN2-3 detection for a sensitivity established at 70% was 44.7% (95%confidence interval [CI] 36.5-53.2) for TOP2A and MKI67 and 54.5% (95%CI 46.0-62.8%) for CDKN2A/p16. mRNA detection of cell biomarkers in anal liquid-based cytology is feasible. Further studies are warranted to confirm if strategies based on mRNA detection have any role in anal cancer screening.15 p.application/pdfengcc-by (c) Pino Saladrigues, Marta del et al., 2019http://creativecommons.org/licenses/by/3.0/esCàncer colorectalCitologiaRNAMarcadors bioquímicsColorectal cancerCytologyRNABiochemical markersinfo:eu-repo/semantics/article6956402020-06-11info:eu-repo/semantics/openAccess31684110