Rodríguez Lagunas, María JoséFerrer i Roig, RuthMoreno Aznárez, Juan José2024-02-212024-02-212013-05-010952-3278https://hdl.handle.net/2445/207865<p>Prostaglandins (PG) are inflammatory mediators derived from arachidonic or eicosapentaenoic acid giving rise to the 2-series or the 3-series prostanoids, respectively. Previously, we have observed that PGE 2 disrupts epithelial barrier function. Considering the beneficial effect of fish oil consumption in intestinal inflammatory processes, the aim of this study was to assess the role of PGE 3 on epithelial barrier function assessed from transepithelial electrical resistance and dextran fluxes in Caco-2 cells. The results indicate that PGE 3 increased paracellular permeability (PP) to the same extent as PGE 2, through the interaction with EP 1 and EP 4 receptors and with intracellular Ca 2+ and cAMP as the downstream targets. Moreover, we observed a redistribution of tight junction proteins, occludin and claudin-4. In conclusion, PGE 3 is able to increase PP thus leading to reconsider the role of PGE 2/PGE 3 ratio in the beneficial effects of dietary fish oil supplementation in the disruption of barrier function.</p>7 p.application/pdfeng(c) International Society for the Study of Fatty Acids and Lipids, 2013Malalties inflamatòries intestinalsEpiteliCèl·lules epitelialsInflammatory bowel diseasesEpitheliumEpithelial cellsinfo:eu-repo/semantics/article6299132024-02-21info:eu-repo/semantics/openAccess