Pinto, DianaLópez-Yerena, AnallelyLamuela Raventós, Rosa Ma.Vallverdú i Queralt, AnnaDelerue-Matos, CristinaRodrigues, Francesca2024-02-272024-02-272024-03-010308-8146https://hdl.handle.net/2445/208113<p>Chestnut (Castanea sativa) shells (CS) are an undervalued antioxidant-rich by-product. This study explores the impact of in-vitro digestion on the bioaccessibility, bioactivity, and metabolic profile of CS extract prepared by Supercritical Fluid Extraction, aiming its valorization for nutraceutical applications. The results demonstrated significantly (p <0.05) lower phenolic concentrations retained after digestion (38.57 µg gallic acid equivalents/ mg dry weight (DW)), reaching 30% of bioaccessibility. The CS extract showed antioxidant/antiradical, hypoglycemic, and neuroprotective properties after in-vitro digestion, along with upmodulating effects on antioxidant enzymes activities and protection against lipid peroxidation. The metabolic profile screened by LC-ESI-LTQ- Orbitrap-MS proved the biotransformation of complex phenolic acids, flavonoids, and tannins present in the undigested extract (45.78 µg/mg DW of total phenolic concentration) into hydroxybenzoic, phenylpropanoic, and phenylacetic acids upon digestion (35.54 µg/mg DW). These findings sustain the valorization of CS extract as a promising nutraceutical ingredient, delivering polyphenols with proven bioactivity even after in-vitro digestion.</p>1 p.application/pdfengcc-by-nc-nd (c) Diana Pinto, et al., 2024http://creativecommons.org/licenses/by-nc-nd/4.0/MetabolòmicaPolifenolsMetabolomicsPolyphenolsinfo:eu-repo/semantics/article7414082024-02-27info:eu-repo/semantics/openAccess