Pena-Rodríguez, EloyLajarin Reinares, MariaMata i Ventosa, AidaPerez Torras, SandraFernández Campos, Francisco2023-04-132023-04-132021-04-111999-4923https://hdl.handle.net/2445/196724Follicular targeting has gained more attention in recent decades, due to the possibility of obtaining a depot effect in topical administration and its potential as a tool to treat hair follicle-related diseases. Lipid core ethyl cellulose lipomers were developed and optimized, following which characterization of their physicochemical properties was carried out. Dexamethasone was encapsulated in the lipomers (size, 115 nm; polydispersity, 0.24; zeta-potential (Z-potential), +30 mV) and their in vitro release profiles against dexamethasone in solution were investigated by vertical diffusion Franz cells. The skin biodistribution of the fluorescent-loaded lipomers was observed using confocal microscopy, demonstrating the accumulation of both lipomers and fluorochromes in the hair follicles of pig skin. To confirm this fact, immunofluorescence of the dexamethasone-loaded lipomers was carried out in pig hair follicles. The anti-inflammatory (via TNFα) efficacy of the dexamethasone-loaded lipomers was demonstrated in vitro in an HEK001 human keratinocytes cell culture and the in vitro cytotoxicity of the nanoformulation was investigated.19 p.application/pdfengcc-by (c) Pena Rodriguez, Eloy et al., 2021https://creativecommons.org/licenses/by/4.0/PellNanopartículesSkinNanoparticlesinfo:eu-repo/semantics/article7176702023-04-13info:eu-repo/semantics/openAccess