Nadal Rodríguez, PauHartung, FrederickPedrola Teixell, MarinaCoomar, SeemonDiaz-Moreno, AlejandroHätälä, Anna M.Rolfes, Katharina M.Sánchez-Vera, IsmaelGil, JoanMolins i Grau, EliesViayna Gaza, AntonioHanzl, AlexanderThomä, Nicolas H.Haarmann-Stemmann, ThomasLuque Garriga, F. XavierLavilla Grífols, RodolfoGhashghaei, Ouldouz2025-06-232025-06-232025-04-102374-7951https://hdl.handle.net/2445/221704A multidisciplinary platform is presented to address aryl hydrocarbon receptor (AhR) modulation. A rewired Yonemitsu multicomponent reaction with indole 2-carboxaldehydes and nucleophilic species was designed to access a family of 6-substituted indolocarbazoles. The conformational behavior of these compounds was examined to rationalize their axial chirality. In silico docking and molecular simulations highlighted key features implicated in their binding to AhR. Furthermore, the synthesis of linkable derivatives allowed the direct development of conjugated entities. Reporter gene and target gene expression analyses identified these novel structures as potent noncytotoxic activating AhR ligands, that can be extended to bifunctional molecules. The anti-inflammatory properties of these AhR agonists were assessed in interleukin-13 treated keratinocytes. Altogether, the synergistic research in synthetic and computational chemistry integrated with biological studies opens novel avenues toward understanding the biological roles of AhR and the development of targeted therapeutics.13 p.application/pdfengcc by (c) Pau Nadal Rodríguez, et al., 2025http://creativecommons.org/licenses/by/3.0/es/Química farmacèuticaSíntesi orgànicaCompostos heterocíclicsPharmaceutical chemistryOrganic synthesisHeterocyclic compoundsinfo:eu-repo/semantics/article7580422025-06-23info:eu-repo/semantics/openAccess