Rico Pastó, MarcRitort Farran, Fèlix2023-05-192023-05-192022-09-142667-0747https://hdl.handle.net/2445/198245Knowledge of the elastic properties, e.g., the persistence length or interphosphate distance, of single-stranded (ss) and double-stranded (ds) DNA under different experimental conditions is critical to characterizing molecular reactions studied with single-molecule techniques. While previous experiments have addressed the dependence of the elastic parameters upon varying ionic strength and contour length, temperature-dependent effects are less studied. Here, we examine the temperature-dependent elasticity of ssDNA and dsDNA in the range 5°C-50°C using a temperature-jump optical trap. We find a temperature softening for dsDNA and a temperature stiffening for ssDNA. Our results highlight the need for a general theory explaining the phenomenology observed.application/pdfengcc-by (c) Rico Pastó, Marc et al., 2022https://creativecommons.org/licenses/by/4.0/ADNNanotecnologiaElasticitatDNANanotechnologyElasticityinfo:eu-repo/semantics/article7316732023-05-19info:eu-repo/semantics/openAccess