Sánchez Velázquez, PatriciaCastellví, QuimVillanueva Garatachea, AlbertoIglesias, MarQuesada, RitaPañella, ClaraCáceres, M.Dorcaratto, D.Andaluz, AnnaMoll, XavierBurdío, J. M.Grande, LuísIvorra, AntoniBurdío, Fernando2018-09-102018-09-102017-03-22https://hdl.handle.net/2445/124412Irreversible electroporation (IRE) has recently gained in popularity as an ablative technique, however little is known about its oncological long-term outcomes. To determine the long-time survival of animals treated with a high dose of IRE and which histological changes it induces in tumoral tissue, IRE ablation was performed in forty-six athymic-nude mice with KM12C tumors implanted in the liver by applying electric current with different voltages (2000 V/cm, 1000 V/cm). The tumors were allowed to continue to grow until the animals reached the end-point criteria. Histology was harvested and the extent of tumor necrosis was semi-quantitatively assessed. IRE treatment with the 2000 V/cm protocol significantly prolonged median mouse survival from 74.3 +/- 6.9 days in the sham group to 112.5 +/- 15.2 days in the 2000 V/cm group. No differences were observed between the mean survival of the 1000 V/cm and the sham group (83.2 +/- 16.4 days, p = 0.62). Histology revealed 63.05% +/- 23.12 of tumor necrosis in animals of the 2000 V/cm group as compared to 17.50% +/- 2.50 in the 1000 V/cm group and 25.6% +/- 22.1 in the Sham group (p = 0.001). IRE prolonged the survival of animals treated with the highest electric field (2000 V/cm). The animals in this group showed significantly higher rate of tumoral necrosis.8 p.application/pdfengcc by (c) Sánchez Velázquez et al., 2017http://creativecommons.org/licenses/by/3.0/es/MetàstasiNecrosiMetastasisNecrosisinfo:eu-repo/semantics/article2018-07-24info:eu-repo/semantics/openAccess28327623