Aguado Tomàs, FernandoGombau, LourdesMajó, GlòriaMarsal Tebé, JordiBlanco Fernández, JerónimoBlasi Cabús, Joan2021-05-252021-05-251997-10-100021-9258https://hdl.handle.net/2445/177587Botulinum neurotoxin type A (BoNT/A) inhibits neurotransmitter release by specific cleavage of SNAP-25, a synaptosome-associated protein also expressed in the ACTH secretory cell line AtT-20. Expression of light chain BoNT/A (L-BoNT/A) gene transfected into AtT-20 cells resulted in a cleaved form of SNAP-25 indistinguishable from that generated by bona fide BoNT/A. L-BoNT/A-transfected cells showed no difference in replication rate, viability, or phenotype, compared with control AtT-20 cells. In contrast, L-BoNT/A-transfected cells could not be induced to secrete ACTH upon stimulation by 8-bromo-cAMP or KCl. In addition, alpha-latrotoxin induced ACTH release from control cells, but not from L-BoNT/A-transfected cells. These experiments suggest an important role for SNAP-25 in regulated secretion from AtT-20 cells and underline the usefulness of this cell system as a tool for the study of the molecular mechanism of peptide hormone secretion.4 p.application/pdfeng(c) American Society for Biochemistry and Molecular Biology, 1997Toxina botulínicaProteïnes de membranaTeixit nerviósHormones pituïtàriesBotulinum toxinMembrane proteinsNerve tissuePituitary hormonesinfo:eu-repo/semantics/article1303662021-05-25info:eu-repo/semantics/openAccess9325336