Viñas Muñoz, José LuisVentayol Espinazo, MarinaBrüne, Bernhard, 1957-Jung, MichaelaSola Martínez, AnnaPi i Siqués, FelipMastora, ChrysoulaHotter Corripio, Georgina2018-01-292018-01-292013-04-101932-6203https://hdl.handle.net/2445/119377This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3β(P) and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production.8 p.application/pdfengcc-by (c) Viñas Muñoz, José Luis et al., 2013http://creativecommons.org/licenses/by/3.0/esCèl·lules mare embrionàriesMicro RNAsDiferenciació cel·lularEmbryonic stem cellsMicroRNAsCell diferentiationinfo:eu-repo/semantics/article6217212018-01-29info:eu-repo/semantics/openAccess23593353