Merino Montero, SusanaBouamama, LamiaaKnirel, Yuriy A.Shenchenkova, Sofya N.Regué Queralt, Miguel, 1953-Tomàs Magaña, Juan2015-01-192015-01-192012-05-011932-6203https://hdl.handle.net/2445/61471We previously reported that A. hydrophila GalU mutants were still able to produce UDP-glucose introduced as a glucose residue in their lipopolysaccharide core. In this study, we found the unique origin of this UDP-glucose from a branched α-glucan surface polysaccharide. This glucan, surface attached through the O-antigen ligase (WaaL), is common to the mesophilic Aeromonas strains tested. The Aeromonas glucan is produced by the action of the glycogen synthase (GlgA) and the UDP-Glc pyrophosphorylase (GlgC), the latter wrongly indicated as an ADP-Glc pyrophosphorylase in the Aeromonas genomes available. The Aeromonas glycogen synthase is able to react with UDP or ADP-glucose, which is not the case of E. coli glycogen synthase only reacting with ADP-glucose. The Aeromonas surface glucan has a role enhancing biofilm formation. Finally, for the first time to our knowledge, a clear preference on behalf of bacterial survival and pathogenesis is observed when choosing to produce one or other surface saccharide molecules to produce (lipopolysaccharide core or glucan).11 p.application/pdfengcc-by (c) Merino Montero, Susana et al., 2012http://creativecommons.org/licenses/by/3.0/esBacterisGlucosaPolisacàridsEnzimsReacció en cadena de la polimerasaBacteriaGlucosePolysaccharidesEnzymesPolymerase chain reactioninfo:eu-repo/semantics/article6145502015-01-15info:eu-repo/semantics/openAccess22563467