García, Juan IgnacioKelley, Holden V.Meléndez, JohannaAlvarez de León, Rosa AlejandraCastillo, AlejandraSidiki, SabeenYusoof, Kizil A.Nunes, ElizabeteLópez Téllez, CesarMejía Villatoro, Carlos RodolfoIkeda, JanetGarcía-Basteiro, Alberto L.Wang, Shu-HuaTorrelles, Jordi B.2020-01-082020-01-082019-11-292045-2322https://hdl.handle.net/2445/147228Tuberculosis (TB) disease still kills 1-person every 21-seconds. Few TB diagnostic tests are considered truly appropriate for point of care settings. The WHO-endorsed immunodiagnostic Alere Determine Lipoarabinomannan Ag-test (LAM-test) detects Mycobacterium tuberculosis complex LAM in urine, and its use is recommended for TB diagnosis among HIV co-infected individuals with low CD4 T-cell counts. Here we found that a simple 15-minute enzymatic treatment at room temperature of LAM-spiked urine with \xCE\xB1-mannosidase (for human TB), and LAM-spiked milk with combined lactase and caseinase (for bovine TB), enhanced 10-fold the detection levels of the LAM-test and thus, improved the detection of LAM by the LAM-test in urine and milk that otherwise could be missed in the field. Future separate clinical research studies specifically designed to address the potential of these findings are required.6 p.application/pdfengcc by (c) García et al., 2019http://creativecommons.org/licenses/by/3.0/es/TuberculosiDiagnòstic immunològicTuberculosisImmunodiagnosisinfo:eu-repo/semantics/article2019-12-20info:eu-repo/semantics/openAccess31784649