Turapov, ObolbekWaddell, Simon J.Burke, BernardGlenn, SarahSarybaeva, Asel A.Tudó i Vilanova, GriseldaLabesse, GillesYoung, Danielle I.Young, MichaelAndrew, Peter W.Butcher, Philip D.Cohen Gonsaun, MartinMukamolova, Galina2015-01-292015-01-292014-07-100021-9258https://hdl.handle.net/2445/62005We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.9 p.application/pdfengcc by (c) Turapov et al., 2014http://creativecommons.org/licenses/by/3.0/Mycobacterium tuberculosisÀcid oleicAntibiòticsMycobacterium tuberculosisOleic acidAntibioticsinfo:eu-repo/semantics/article6434422015-01-29info:eu-repo/semantics/openAccess25012658