Breen, Michael P., 1940-Camps Camprubí, MartaCarvalho-Simoes, FranciscoZorzano Olarte, AntonioPilch, Paul F.2018-09-172018-09-172012-04-061932-6203https://hdl.handle.net/2445/124628Caveolae, little caves of cell surfaces, are enriched in cholesterol, a certain level of which is required for their structural integrity. Here we show in adipocytes that cavin-2, a peripheral membrane protein and one of 3 cavin isoforms present in caveolae from non-muscle tissue, is degraded upon cholesterol depletion in a rapid fashion resulting in collapse of caveolae. We exposed 3T3-L1 adipocytes to the cholesterol depleting agent methyl-β-cyclodextrin, which results in a sudden and extensive degradation of cavin-2 by the proteasome and a concomitant movement of cavin-1 from the plasma membrane to the cytosol along with loss of caveolae. The recovery of cavin-2 at the plasma membrane is cholesterol-dependent and is required for the return of cavin-1 from the cytosol to the cell surface and caveolae restoration. Expression of shRNA directed against cavin-2 also results in a cytosolic distribution of cavin-1 and loss of caveolae. Taken together, these data demonstrate that cavin-2 functions as a cholesterol responsive component of caveolae that is required for cavin-1 localization to the plasma membrane, and caveolae structural integrity.8 p.application/pdfengcc-by (c) Breen, Michael P., 1940- et al., 2012http://creativecommons.org/licenses/by/3.0/esColesterolProteïnes de membranaCèl·lules animalsCholesterolMembrane proteinsAnimal cellsinfo:eu-repo/semantics/article6393352018-09-17info:eu-repo/semantics/openAccess22493697