Balsells Vives, SaraFluegge, UlrikeBrix, BettinaWeimann, YvonnePeralta, TeresaSan Bartolomé, ClaraAraujo Sánchez, GiovannaCasas Saucedo, RocioTorradeflot, MaríaLara, RocíoMuñoz-Cano, RosaBartra Tomàs, JoanSuer, WaltraudPascal, Mariona2024-12-232024-12-232023-06-021613-4125https://hdl.handle.net/2445/217251Scope: LTP-syndrome is characterized by sensitization (IgE) to multiple non-specific lipid transfer proteins (nsLTPs) with a variable clinical outcome. The treatment is primarily based on offending food avoidance. However, the determination of Pru p 3-specific IgE is currently the main diagnostic tool to assess sensitization to nsLTPs. Herein, the study evaluates improvement of LTP-syndrome diagnosis and clinical management using a new IgE multiplex-immunoblot assay with a high diversity of food nsLTPs. Methods and results: An EUROLINE-LTP strip with 28 recombinant nsLTPs from 18 allergenic sources is designed. In total the study investigates 38 patients with LTP-syndrome and compares results from the nsLTPs (LTP-strip) with the respective food extracts of Prick-by-prick (PbP) testing. The agreement exceeds 70% for most nsLTPs, e.g., Pru p 3 (100%), Mal d 3 (97%), Pru av 3 (89%), Pha v 3 isoforms (87%/84%), Ara h 9 (82%), Cor a 8 (82%), and Jug r 3 (82%). The functionality and allergenic relevance of nine recombinant nsLTPs are proven by Basophil activation testing (BAT). Conclusions: The new IgE multiplex-immunoblot nsLTP assay shows a good diagnostic performance allowing culprit food assessment. Negative results from LTP-strip may indicate potentially tolerable foods, improving diet intervention and patients' quality of life.10 p.application/pdfengcc-by-nc-nd (c) Balsells Vives, Sara et al., 2023http://creativecommons.org/licenses/by-nc-nd/4.0/Proteïnes vegetalsImmunoglobulina EAl·lèrgia alimentàriaLípidsAntígensPlant proteinsImmunoglobulin EFood allergyLipidsAntigensinfo:eu-repo/semantics/article7517532024-12-23info:eu-repo/semantics/openAccess37195823