Gállego, IsaacManning, BrendanPrades García, Juan DanielMir Llorente, MònicaSamitier i Martí, JosepEritja i Casadellà, Ramon2020-05-032020-05-032017-08-082504-3900https://hdl.handle.net/2445/158458We report the first DNA-based origami technique that can print addressable patterns on surfaces with sub-10 nm resolution. Specifically, we have used a two-dimensional DNA origami as a template (DNA origami stamp) to transfer DNA with pre-programmed patterns (DNA ink) on gold surfaces. The DNA ink is composed of thiol-modified staple strands incorporated at specific positions of the DNA origami stamp to create patterns upon thiol-gold bond formation on the surface (DNA ink). The DNA pattern formed is composed of unique oligonucleotide sequences, each of which is individually addressable. As a proof-of-concept, we created a linear pattern of oligonucleotide-modified gold nanoparticles complementary to the DNA ink pattern. We have developed an in silico model to identify key elements in the formation of our DNA origami-driven lithography and nanoparticle patterning as well as simulate more complex nanoparticle patterns on surfaces.4 p.application/pdfengcc-by (c) Gállego, Isaac et al., 2017http://creativecommons.org/licenses/by/3.0/esADNLitografiaNanopartículesOrOrigamiDNALithographyNanoparticlesGoldOrigamiinfo:eu-repo/semantics/article6775462020-05-03info:eu-repo/semantics/openAccess