Moro Moreno, VíctorCanals Gil, JoanMoreno Martín, SergioBornemann, SteffenAlonso Casanovas, OscarWaag, AndreasPrades García, Juan DanielDiéguez Barrientos, Àngel2025-04-232025-04-232024-05-212079-6374https://hdl.handle.net/2445/220537A Point-of-Care system for molecular diagnosis (PoC-MD) is described, combining GaN and CMOS chips. The device is a micro-system for fluorescence measurements, capable of analyzing both intensity and lifetime. It consists of a hybrid micro-structure based on a 32 × 32 matrix addressable GaN microLED array, with square LEDs of 50 µm edge length and 100 µm pitch, with an underneath wire bonded custom chip integrating their drivers and placed face-to-face to an array of 16 × 16 single-photon avalanche diodes (SPADs) CMOS. This approach replaces instrumentation based on lasers, bulky optical components, and discrete electronics with a full hybrid micro-system, enabling measurements on 32 × 32 spots. The reported system is suitable for long lifetime (>10 ns) fluorophores with a limit of detection ~1/4 µM. Proof-of-concept measurements of streptavidin conjugate Qdot™ 605 and Amino PEG Qdot™ 705 are demonstrated, along with the device ability to detect both fluorophores in the same measurement17 p.application/pdfengcc-by (c) Moro, V. et al., 2024http://creativecommons.org/licenses/by/4.0/FluorescènciaDíodes electroluminescentsFluorescenceLight emitting diodesinfo:eu-repo/semantics/article7486732025-04-23info:eu-repo/semantics/openAccess