Barbé García, JordiSala, MontserratLlagostera, MontserratGuerrero, Ricardo, 1943-2019-01-162019-01-1619890212-3037https://hdl.handle.net/2445/127322The amino-terminal fragment of the RecA protein was cloned in pUA27 plasmid and introduced in different repair mutants of Escherichia coli. The UV-mediated induction of several SOS functions was then studied. Results show that the truncated RecA protein plays an important role in the UV-damaged DNA when the level of the chromosomal RecA protein is low. Furthermore, RecA protein inhibited the protease activity of the wild type RecA protein causing a decrease in the SOS system induction. All these data suggest that hybrid tetramers between both truncated and wild type RecA proteins may be formed, and as a consequence the ATPase capacity and active conformation of the wild type RecA protein are blocked.14 p.application/pdfcatcc-by-nc-nd (c) Barbé García, Jordi et al., 1989http://creativecommons.org/licenses/by-nc-nd/3.0/esDuplicació de l'ADNEscheríchia coliReparació de l'ADNDNA replicationEscherichia coliDNA repairinfo:eu-repo/semantics/article0521612019-01-16info:eu-repo/semantics/openAccess