Rovira, MeritxellHuang, WeiYusuff, ShamilaShim, Joong SupFerrante, Anthony A.Liu, Jun O.Parsons, Michael J.2023-02-172023-02-172011-11-290027-8424https://hdl.handle.net/2445/193790Pancreatic β-cells are an essential source of insulin and their destruction because of autoimmunity causes type I diabetes. We conducted a chemical screen to identify compounds that would induce the differentiation of insulin-producing β-cells in vivo. To do this screen, we brought together the use of transgenic zebrafish as a model of β-cell differentiation, a unique multiwell plate that allows easy visualization of lateral views of swimming larval fish and a library of clinical drugs. We identified six hits that can induce precocious differentiation of secondary islets in larval zebrafish. Three of these six hits were known drugs with a considerable background of published data on mechanism of action. Using pharmacological approaches, we have identified and characterized two unique pathways in β-cell differentiation in the zebrafish, including down-regulation of GTP production and retinoic acid biosynthesis.6 p.application/pdfeng(c) Rovira, Meritxell et al., 2011Diferenciació cel·lularInsulinaDisseny de medicamentsCell diferentiationInsulinDrug designinfo:eu-repo/semantics/article6994452023-02-17info:eu-repo/semantics/openAccess22084084