Guerrero-Latorre, LauraCollado, NeusAbasolo, NereaAnzaldi, GabrielBofill Mas, SilviaBosch, AlbertBosch, LluísBusquets, SílviaCaimari, AntoniCanela, NúriaCarcereny, AlbertChacón-Villanueva, CarmeCiruela, PilarCorbella, IreneDomingo, XavierEscoté, XavierEspiñeira, YaimaraForés, EvaGandullo-Sarró, IsabelGarcia-Pedemonte, DavidGironès Llop, RosinaGuix Arnau, SusanaHundesa, AyalkibetItarte, MartaMariné-Casado, RogerMartínez, AnnaMartínez-Puchol, SandraMejías-Molina, CristinsMoliner i Rafa, MarcMunné, AntoniPintó Solé, Rosa MaríaPueyo‑Ros, JosepRobusté‑Cartró, JordiRusiñol Arantegui, MartaSanfeliu, RobertTeichenné, JoanTorrell, HelenaCorominas, LluísBorrego, Carles M.2023-03-282023-03-2820222045-2322https://hdl.handle.net/2445/196060Wastewater-based epidemiology has shown to be an efficient tool to track the circulation of SARS-CoV-2 in communities assisted by wastewater treatment plants (WWTPs). The challenge comes when this approach is employed to help Health authorities in their decision-making. Here, we describe the roadmap for the design and deployment of SARSAIGUA, the Catalan Surveillance Network of SARS-CoV-2 in Sewage. The network monitors, weekly or biweekly, 56 WWTPs evenly distributed across the territory and serving 6 M inhabitants (80% of the Catalan population). Each week, samples from 45 WWTPs are collected, analyzed, results reported to Health authorities, and finally published within less than 72 h in an online dashboard (https://sarsaigua.icra.cat). After 20 months of monitoring (July 20-March 22), the standardized viral load (gene copies/day) in all the WWTPs monitored fairly matched the cumulative number of COVID-19 cases along the successive pandemic waves, showing a good fit with the diagnosed cases in the served municipalities (Spearman Rho = 0.69). Here we describe the roadmap of the design and deployment of SARSAIGUA while providing several open-access tools for the management and visualization of the surveillance data.10 p.application/pdfengcc-by (c) Guerrero-Latorre, Laura et al., 2022https://creativecommons.org/licenses/by/4.0/SARS-CoV-2Aigües residualsEpidemiologiaCOVID-19SARS-CoV-2SewageEpidemiologyCOVID-19info:eu-repo/semantics/article7253092023-03-28info:eu-repo/semantics/openAccess