Cerón Madrigal, JuliánAhumada Brena, David Ricardo2023-07-172023-07-172021-07-09https://hdl.handle.net/2445/200760[eng] Mammalian Inhibitor-of-kappa-B (IκBs) proteins exert their main function as negative regulators of NF-κB, a central signaling pathway controlling immunity and inflammation. An alternative chromatin role for IκBs has been shown to affect stemness and cell differentiation. However, the involvement of NF-κB in this function has not been excluded. NFKI-1 and IKB1 are IκB homologs in aenorhabditis elegans, which lacks NF-κB nuclear effectors. We found that nfki-1 and ikb-1 mutants display developmental defects that phenocopy mutations in Polycomb and UTX-1 histone demethylase, suggesting a role for C. elegans IκBs in chromatin regulation. Further supporting this possibility (i) we detected NFKI-1 in the nucleus of cells; (ii) NFKI-1 and IKB-1 bind to histones and Polycomb proteins, (iii) and associate with chromatin in vivo, and (iv) mutations in nfki-1 and ikb-1 alter chromatin marks. Based on these results, we propose that ancestral IκB proteins modulate chromatin marks with an impact on development.Universitat de Barcelona. Facultat de Medicinaapplication/pdfengcc by (c) Ahumada Brena, David Ricardo, 2023http://creativecommons.org/licenses/by/3.0/es/EpigenèticaCàncerProteïnes supressores de tumorsModels animals en la investigacióEpigeneticsCancerTumor suppressor proteinAnimal models in researchinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccesshttp://hdl.handle.net/10803/688664