Detection of heteromers formed by cannabinoid CB1, dopamine D2, and adenosine A2A G-protein-coupled receptors by combining bimolecular fluorescence complementation and bioluminescence energy transfer

Navarro Brugal, GemmaCarriba Domínguez, PaulinaGandía, JorgeCiruela Alférez, FranciscoCasadó, VicentCortés Tejedor, AntonioMallol Montero, JosefaCanela Campos, Enric I. (Enric Isidre), 1949-Lluís i Biset, CarmeFranco Fernández, Rafael2018-05-172018-05-172008-10-111537-744Xhttps://hdl.handle.net/2445/122431Functional interactions in signaling occur between dopamine D2 (D2R) and cannabinoid CB1 (CB1R) receptors, between CB1R and adenosine A2A (A2AR) receptors, and between D2R and A2AR. Furthermore, direct molecular interactions have been reported for the pairs CB1R-D2R, A2AR-D2R, and CB1R-A2AR. Here a combination of bimolecular fluorescence complementation and bioluminescence energy transfer techniques was used to identify the occurrence of D2R-CB1R-A2AR hetero-oligomers in living cells.11 p.application/pdfengcc-by (c) Navarro Brugal, Gemma et al., 2008http://creativecommons.org/licenses/by/3.0/esAdenosinaProteïnesReceptors cel·lularsAdenosineProteinsCell receptorsDetection of heteromers formed by cannabinoid CB1, dopamine D2, and adenosine A2A G-protein-coupled receptors by combining bimolecular fluorescence complementation and bioluminescence energy transferinfo:eu-repo/semantics/article5642412018-05-17info:eu-repo/semantics/openAccess18956124