Hrvolová, BarboraColmán Martínez, Lourdes MarielMartínez Huélamo, MiriamHurtado Barroso, SaraLamuela Raventós, Rosa Ma.Kalina, Jirí2020-03-022020-03-022016-10-141661-6596https://hdl.handle.net/2445/151684The concentration of carotenoids and fat-soluble vitamins in human plasma may play a significant role in numerous chronic diseases such as age-related macular degeneration and some types of cancer. Although these compounds are of utmost interest for human health, methods for their simultaneous determination are scarce. A new high pressure liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) method for the quantification of selected carotenoids and fat-soluble vitamins in human plasma was developed, validated, and then applied in a pilot dietary intervention study with healthy volunteers. In 50 min, 16 analytes were separated with an excellent resolution and suitable MS signal intensity. The proposed HPLC-MS/MS method led to improvements in the limits of detection (LOD) and quantification (LOQ) for all analyzed compounds compared to the most often used HPLC-DAD methods, in some cases being more than 100-fold lower. LOD values were between 0.001 and 0.422 µg/mL and LOQ values ranged from 0.003 to 1.406 µg/mL, according to the analyte. The accuracy, precision, and stability met with the acceptance criteria of the AOAC (Association of Official Analytical Chemists) International. According to these results, the described HPLC-MS/MS method is adequately sensitive, repeatable and suitable for the large-scale analysis of compounds in biological fluids.17 p.application/pdfengcc-by (c) Hrvolová, Barbora et al., 2016http://creativecommons.org/licenses/by/3.0/esCarotenoidesVitamines en la nutrició humanaPlasma sanguiniCarotenoidsVitamins in human nutritionBlood plasmainfo:eu-repo/semantics/article6688712020-03-02info:eu-repo/semantics/openAccess27754400