García-Negredo, GloriaSoto del Cerro, DavidLlorente, JavierMorató Arús, XavierGalenkamp, Koen M.O.Gómez Soler, MaricelFernández Dueñas, VíctorWatanabe, MasahikoAdelman, John P.Shigemoto, RyuichiFukazawa, YugoLuján, RafaelCiruela Alférez, Francisco2017-05-042017-05-042014-10-290270-6474https://hdl.handle.net/2445/110442Group I metabotropic glutamate (mGlu) receptors regulate hippocampal CA1 pyramidal neuron excitability via Ca(2+) wave-dependent activation of small-conductance Ca(2+)-activated K(+) (SK) channels. Here, we show that mGlu5 receptors and SK2 channels coassemble in heterologous coexpression systems and in rat brain. Further, in cotransfected cells or rat primary hippocampal neurons, mGlu5 receptor stimulation activated apamin-sensitive SK2-mediated K(+) currents. In addition, coexpression of mGlu5 receptors and SK2 channels promoted plasma membrane targeting of both proteins and correlated with increased mGlu5 receptor function that was unexpectedly blocked by apamin. These results demonstrate a reciprocal functional interaction between mGlu5 receptors and SK2 channels that reflects their molecular coassembly.10 p.application/pdfengcc-by-nc-sa (c) García-Negredo, Gloria et al., 2014http://creativecommons.org/licenses/by-nc-sa/3.0/esCanals de potassiNeurologiaHipocamp (Cervell)FarmacologiaRates (Animals de laboratori)Potassium channelsNeurologyHippocampus (Brain)PharmacologyRats as laboratory animalsinfo:eu-repo/semantics/article6436222017-05-04info:eu-repo/semantics/openAccess25355231