Hereu, MercèRamos Romero, SaraGarcía-González, NataliaAmézqueta, SusanaTorres, Josep Lluís2020-05-252020-05-252018-09-161756-4646https://hdl.handle.net/2445/162310Diversity and balance of gut microorganisms is fundamental for health throughout life. The aim of this study is to explore the possible eubiotic effect of the buckwheat iminosugar D-fagomine (0.096% w/w in standard feed) in growing healthy Wistar Kyoto rats. Feed and energy intake, residual energy in feces, and body weight gain were independent of D-fagomine supplementation throughout the intervention (24 weeks). The populations of significant bacterial subgroups and species were determined in fecal and cecal DNA by quantitative real-time PCR. D-Fagomine increased the Bacteroidetes:Firmicutes ratio and partially counteracted the loss of Lactobacilliales and Bifidobacteriales over time. The supplementation reduced the levels of excreted short- chain fatty acids (SCFAs) as determined by gas chromatography. This paper provides preliminary evidence that D-fagomine has the capacity to promote microbial functional diversity by increasing the Bacteroidetes:Firmicutes ratio and to mitigate the age-related reduction in populations of the putatively beneficial Lactobacilliales and Bifidobacteriales.7 p.application/pdfengcc-by-nc-nd (c) Hereu, Mercè et al., 2018http://creativecommons.org/licenses/by-nc-nd/3.0/esLactobacilsMicrobiotaBifidobacterisLactobacillusMicrobiotaBifidobacteriuminfo:eu-repo/semantics/article6837222020-05-25info:eu-repo/semantics/openAccess