Olmeda, DavidCastel i Gil, SusannaVilaró, Senén, 1956-2005Cano, Amparo2012-05-092012-05-092003-03-111059-1524https://hdl.handle.net/2445/25182ß-catenin is a multifunctional protein involved in cell-cell adhesion and Wnt signal transduction. ß-Catenin signaling has been proposed to act as inducer of cell proliferation in different tumors. However, in some developmental contexts and cell systems ß-catenin also acts as a positive modulator of apoptosis. To get additional insights into the role of ß-Catenin in the regulation of the cell cycle and apoptosis, we have analyzed the levels and subcellular localization of endogenous ß-catenin and its relation with adenomatous polyposis coli (APC) during the cell cycle in S-phase¿synchronized epithelial cells. ß-Catenin levels increase in S phase, reaching maximum accumulation at late G2/M and then abruptly decreasing as the cells enter into a new G1 phase. In parallel, an increased cytoplasmic and nuclear localization of ß-catenin and APC is observed during S and G2 phases. In addition, strong colocalization of APC with centrosomes, but not ß-catenin, is detected in M phase. Interestingly, overexpression of a stable form of ß-catenin, or inhibition of endogenous ß-catenin degradation, in epidermal keratinocyte cells induces a G2 cell cycle arrest and leads to apoptosis. These results support a role for ß-catenin in the control of cell cycle and apoptosis at G2/M in normal and transformed epidermal keratinocytes.17 p.application/pdfengcc-by-nc-sa, (c) Olmeda et al., 2003http://creativecommons.org/licenses/cc-by-nc-sa/3.0/esApoptosiCicle cel·lularProteïnes citosquelètiquesApoptosisCell cycleCytoskeletal proteinsinfo:eu-repo/semantics/article520340info:eu-repo/semantics/openAccess12857869