Navarro Otano, JudithMorén Núñez, ConstanzaGonzález Casacuberta, IngridJuárez Flores, Diana LuzVilas Rolán, DoloresGarrabou Tornos, GlòriaMilisenda, JoséPont Sunyer, ClaustreCatalán García, MarcGuitart Mampel, MarionaTobías, EsterCardellach, FrancescValldeoriola Serra, FrancescIranzo, AlexTolosa, Eduardo2025-02-202025-02-202017-06-042042-0080https://hdl.handle.net/2445/219047Objective: To determine potential mitochondrial and oxidative alterations in colon biopsies from idiopathic REM sleep behavior disorder (iRBD) and Parkinson's disease (PD) subjects. Methods: Colonic biopsies from 7 iRBD subjects, 9 subjects with clinically diagnosed PD, and 9 healthy controls were homogenized in 5% w/v mannitol. Citrate synthase (CS) and complex I (CI) were analyzed spectrophotometrically. Oxidative damage was assessed either by lipid peroxidation, through malondialdehyde and hydroxyalkenal content by spectrophotometry, or through antioxidant enzyme levels of superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (Gpx1), and catalase (CAT) by western blot. The presence of mitochondrial DNA (mtDNA) deletions was assessed by long PCR and electrophoresis. Results: Nonsignificant trends to CI decrease in both iRBD (45.69 ± 18.15; 23% decrease) and PD patients (37.57 ± 12.41; 37% decrease) were found compared to controls (59.51 ± 12.52, p: NS). Lipid peroxidation was maintained among groups (iRBD: 27.46 ± 3.04, PD: 37.2 ± 3.92, and controls: 31.71 ± 3.94; p: NS). Antioxidant enzymes SOD2 (iRBD: 2.30 ± 0.92, PD: 1.48 ± 0.39, and controls: 1.09 ± 0.318) and Gpx1 (iRBD 0.29 ± 0.12, PD: 0.56 ± 0.33, and controls: 0.38 ± 0.16) did not show significant differences between groups. CAT was only detected in 2 controls and 1 iRBD subject. One iRBD patient presented a single mtDNA deletion.7 p.application/pdfengcc-by (c) Morén, C. et al., 2017http://creativecommons.org/licenses/by/4.0/Malaltia de ParkinsonBiòpsiaCòlonTrastorns del sonParkinson's diseaseBiopsyColonSleep disordersinfo:eu-repo/semantics/article6772072025-02-20info:eu-repo/semantics/openAccess