Bonelli Blasco, Joaquin DanielVelasco de Andrés, MaríaIsidro, NeusBayó, CristinaChumillas, SergiCarrillo-Serradell, LauraCasadó Llombart, SergiMok, CherylBenítez-Ribas, DanielLozano Soto, FranciscoRocas Sorolla, JosepMarchán Sancho, Vicente2023-04-282023-04-282022-12-241999-4923https://hdl.handle.net/2445/197404Encapsulation of water-soluble bioactive compounds for enabling specific accumulation in tumor locations, while avoiding premature clearance and/or degradation in the bloodstream, is one of the main hallmarks in nanomedicine, especially that of NIR fluorescent probes for cancer theragnosis. The herein reported technology furnishes water-dispersible double-walled polyurethane-polyurea hybrid nanocapsules (NCs) loaded with indocyanine green (ICG-NCs), using a versatile and highly efficient one-pot and industrially scalable synthetic process based on the use of two different prepolymers to set up the NCs walls. Flow cytometry and confocal microscopy confirmed that both ICG-loaded NCs internalized in monocyte-derived dendritic cells (moDCs). The in vivo analysis of xenograft A375 mouse melanoma model revealed that amphoteric functionalization of NCs' surface promotes the selective accumulation of ICG-NCs in tumor tissues, making them promising agents for a less-invasive theragnosis of cancer.application/pdfengcc-by (c) Bonelli Blasco, Joaquin Daniel et al., 2022https://creativecommons.org/licenses/by/4.0/BiocompatibilitatTumorsPoliuretansBiocompatibilityTumorsPolyurethanesinfo:eu-repo/semantics/article7296442023-04-28info:eu-repo/semantics/openAccess