Benito, S.Ferrer, AssumptaBenabou Zdaou, SanaeAviñó Andrés, AnnaEritja i Casadellà, RamonGargallo Gómez, Raimundo2018-02-132020-02-062018-02-061386-1425https://hdl.handle.net/2445/119785Guanine-rich sequences may fold into highly ordered structures known as G-quadruplexes. Apart from the monomeric G-quadruplex, these sequences may form multimeric structures that are not usually considered when studying interaction with ligands. This work studies the interaction of a ligand, crystal violet, with three guanine-rich DNA sequences with the capacity to form multimeric structures. These sequences correspond to short stretches found near the promoter regions of c-kit and SMARCA4 genes. Instrumental techniques (circular dichroism, molecular fluorescence, size-exclusion chromatography and electrospray ionization mass spectrometry) and multivariate data analysis were used for this purpose. The polymorphism of G-quadruplexes was characterized prior to the interaction studies. The ligand was shown to interact preferentially with the monomeric G-quadruplex; the binding stoichiometry was 1:1 and the binding constant was in the order of 105 M-1 for all three sequences. The results highlight the importance of DNA treatment prior to interaction studies42 p.application/pdfengcc-by-nc-nd (c) Elsevier B.V., 2018http://creativecommons.org/licenses/by-nc-nd/3.0/esPolimorfisme (Cristal·lografia)Anàlisi multivariableLligandsG-estructuresPolymorphism (Crystallography)Multivariate analysisLigandsG-structuresinfo:eu-repo/semantics/article6761322018-02-13info:eu-repo/semantics/openAccess29448171