Hillion, JoëlleCanals Buj, MeritxellTorvinen, MariaCasadó, VicentScott, RizaldyTerasmaa, AntonHansson, AnitaWatson, StanleyOlah, Mark E.Mallol Montero, JosefaCanela Campos, Enric I. (Enric Isidre), 1949-Zoli, MicheleAgnati, Luigi F.Ibanez, Carlos F.Lluís i Biset, CarmeFranco Fernández, RafaelFerré, SergiFuxe, Kjell2018-05-112018-05-112002-050021-9258https://hdl.handle.net/2445/122309Antagonistic and reciprocal interactions are known to exist between adenosine and dopamine receptors in the striatum. In the present study, double immunofluorescence experiments with confocal laser microscopy showed a high degree of colocalization of adenosine A(2A) receptors (A(2A)R) and dopamine D(2) receptors (D(2)R) in cell membranes of SH-SY5Y human neuroblastoma cells stably transfected with human D(2)R and in cultured striatal cells. A(2A)R/D(2)R heteromeric complexes were demonstrated in coimmunoprecipitation experiments in membrane preparations from D(2)R-transfected SH-SY5Y cells and from mouse fibroblast Ltk(-) cells stably transfected with human D(2)R (long form) and transiently cotransfected with the A(2A)R double-tagged with hemagglutinin. Long term exposure to A(2A)R and D(2)R agonists in D(2)R-cotransfected SH-SY5Y cells resulted in coaggregation, cointernalization and codesensitization of A(2A)R and D(2)R. These results give a molecular basis for adenosine-dopamine antagonism at the membrane level and have implications for treatment of Parkinson's disease and schizophrenia, in which D(2)R are involved.8 p.application/pdfeng(c) American Society for Biochemistry and Molecular Biology, 2002Receptors cel·lularsMalalties del sistema nerviósAdenosinaCell receptorsNervous system DiseasesAdenosineinfo:eu-repo/semantics/article5015952018-05-11info:eu-repo/semantics/openAccess