Trueba-Santiso, AlbaTorrentó, ClaraSoder-Walz, Jesica M.Fernández-Verdejo, DavidRosell, MònicaMarco-Urrea, Ernest2024-10-142024-10-142024-06-010045-6535https://hdl.handle.net/2445/2157271,2-dichloropropane (1,2-DCP) and 1,2,3-trichloropropane (1,2,3-TCP) are hazardous chemicals frequently detected in groundwater near agricultural zones due to their historical use in chlorinated fumigant formulations. In this study, we show that the organohalide-respiring bacterium <em style="color:black">Dehalogenimonas alkenigignens</em><span style="color:black"> strain BRE15M can grow during the dihaloelimination of </span>1,2-DCP and 1,2,3-TCP to propene and allyl chloride, respectively. Our work also provides the first application of dual isotope approach to investigate the anaerobic reductive dechlorination of 1,2-DCP and 1,2,3-TCP. Stable carbon and chlorine isotope fractionation values for 1,2-DCP (Ɛ<sub>C </sub>= -13.6 ± 1.4 ‰ and Ɛ<sub>Cl</sub>= -27.4 ± 5.2 ‰) and 1,2,3-TCP (Ɛ<sub>C </sub>= -3.8 ± 0.6 ‰ and Ɛ<sub>Cl</sub>= -0.8 ± 0.5 ‰) were obtained resulting in distinct dual isotope slopes (Λ<sub>12DCP</sub>= 0.5 ± 0.1, Λ<sub>123TCP</sub>= 4 ± 2). However direct comparison of Λ<sub>C-Cl</sub> among different substrates is not possible and investigation of the C and Cl apparent kinetic isotope effects lead to the hypothesis that <em>concerted </em>dichloroelimination mechanism is more likely for both compounds. In fact, whole cell activity assays using cells suspensions of the <em>Dehalogenimonas</em>-containing culture grown with 1,2-DCP and methyl viologen as electron donor suggest that the same set of reductive dehalogenases was involved in the transformation of 1,2-DCP and 1,2,3-TCP. This study opens the door to the application of isotope techniques for evaluating biodegradation of 1,2-DCP and 1,2,3-TCP, which often co-occur in groundwaters near agricultural fields.11 p.application/pdfengcc-by-nc-nd (c) The Author(s), 2024http://creativecommons.org/licenses/by-nc-nd/4.0/Hidrologia d'aigües subterràniesContaminació de l'aiguaIsòtopsGroundwater hydrologyWater pollutionIsotopesDual C–Cl isotope fractionation offers potential to assess biodegradation of 1,2-dichloropropane and 1,2,3-trichloropropane by Dehalogenimonas culturesinfo:eu-repo/semantics/article7483222024-10-14info:eu-repo/semantics/openAccess