Puig Pitarch, CarmenMartí Martí, SaraFleites, AnaTrabazo, RafaelCalatayud, LauraLiñares Louzao, JosefinaArdanuy Tisaire, María Carmen2015-07-132015-07-132014-101076-6294https://hdl.handle.net/2445/66315Haemophilus influenzae colonizes the upper respiratory tract and can spread causing otitis and sinusitis. This work aimed to study the oropharyngeal carriage rate in healthy <5-year-old children attending day care centers in Oviedo, Spain in two consecutive years (January to March 2004-2005). The carriage rate was 42% (400/960) and highly variable among centers (range, 12% to 83%). Isolates were mainly identified as nontypeable H. influenzae (NTHi, 99%). Epidemiologically, 127 different genotypes were identified by PFGE with a minimum of two genotypes per center. One hundred fourteen children (12%) were included in both studies and none of them harbored the same strain over a period of time. The isolates only showed resistance to cotrimoxazol and ampicillin, presenting a shift in the level of ampicillin reduced susceptibility, showing a predominance of PBP3 mutations in 2004 and a predominance of β-lactamase production in 2005. This study proved the great genetic variability of NTHi isolates that present similar genotypic patterns in both years with no long-term carriage of the same strain.6 p.application/pdfeng(c) Mary Ann Liebert, Inc., 2014Infeccions en els infantsMalalties dels infantsBacilsFaringeEscoles bressolInfection in childrenChildren's diseasesBacillus (Bacteria)PharynxKindergarteninfo:eu-repo/semantics/article6523472015-07-13info:eu-repo/semantics/openAccess24716536