Colomer, JordiLozano Gargallo, Clàudia2024-06-142024-06-142024-06-05https://hdl.handle.net/2445/213223Treballs Finals de Grau d'Enginyeria Biomèdica. Facultat de Medicina i Ciències de la Salut. Universitat de Barcelona. Curs: 2023-2024. Tutor/Director: Jordi Colomer ; Director: Jordi Abantebrain regions,particularlyaffectingthestriatalneuronsknownasMediumSpinyNeurons(MSNs). Unfortunately,neuronsdonotregenerate,thus,makingitimpossibleforthebodytoreplacetheir function. Despiteextensiveresearch,thereiscurrentlynocureforHD,andthegoaloftreatmentis to alleviatesymptoms. Stem celltherapyrepresentsapromisingdirection,allowingindefinite in vitro cells expansionand differentiationintothedesiredtargetneurons.Themainlimitationofneuralcellstransplantisthat once theirprojectionsaregrown,theycannotbedetractedfromthecultureplatewithoutbreaking and causingcelldeath. In thisproject,wefocusonidentifyingtheoptimaltargetprogenitorsubpopulation.Thecellsshould be matureenoughtoexclusivelydifferentiateintoMSNsbutyetnotoverlymature,preventingthe development oftheprojectionsthatleadtocelldeathuponbreaking.Leveragingcomputational genomics, weanalyzehumansingle-cellRNAsequencingdatatocharacterizetheMSNdifferen- tiation process.Integrating in vivo and in vitro data, wegroupcellsindifferentclustersdepending on theirgeneticexpressionandcomputetheirvelocitiestoanalysetheirfate.Ourfindingsreveala potential groupofcellsthatseemtofulfillallthecriteria.Additionally,theprojecthasenabledthe characterization ofthedifferentlineagesofcellswithinthedifferentiationprocessofstriatalneurons.72 p.application/pdfengcc-by-nc-nd (c) Clàudia Lozano Gargallo, 2024http://creativecommons.org/licenses/by-nc-nd/3.0/es/Enginyeria biomèdicaMaterials biomèdicsTreballs de fi de grauBiomedical engineeringBiomedical materialsBachelor's thesesinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/openAccess