Roig-Soriano, JoanSánchez de Diego, CristinaEsandi-Jauregui, JonVerdés, SergiAbraham, CarmelaBosch, AssumpcióVentura Pujol, FrancescChillón, Miguel2023-04-262023-04-262023-03-142045-2322https://hdl.handle.net/2445/197290The aging-protective gene α-Klotho (KL) produces two main transcripts. The full-length mRNA generates a transmembrane protein that after proteolytic ectodomain shedding can be detected in serum as processed Klotho (p-KL), and a shorter transcript which codes for a putatively secreted protein (s-KL). Both isoforms exhibit potent pleiotropic beneficial properties, although previous reports showed negative side effects on mineral homeostasis after increasing p-KL concentration exogenously. Here, we expressed independently both isoforms using gene transfer vectors, to assess s-KL effects on mineral metabolism. While mice treated with p-KL presented altered expression of several kidney ion channels, as well as altered levels of Pi and Ca2+ in blood, s-KL treated mice had levels comparable to Null-treated control mice. Besides, bone gene expression of Fgf23 showed a fourfold increase after p-KL treatment, effects not observed with the s-KL isoform. Similarly, bone microstructure parameters of p-KL-treated mice were significantly worse than in control animals, while this was not observed for s-KL, which showed an unexpected increase in trabecular thickness and cortical mineral density. As a conclusion, s-KL (but not p-KL) is a safe therapeutic strategy to exploit KL anti-aging protective effects, presenting no apparent negative effects over mineral metabolism and bone microstructure.10 p.application/pdfengcc-by (c) Roig-Soriano, Joan et al., 2023https://creativecommons.org/licenses/by/4.0/OssosRonyóAnimalsRatolins (Animals de laboratori)BonesKidneyAnimalsMice (Laboratory animals)info:eu-repo/semantics/article7334822023-04-26info:eu-repo/semantics/openAccess36918615