Castillo-Lara, SergioAbril Ferrando, Josep Francesc, 1970-2023-03-152023-03-152017-11-241367-4803https://hdl.handle.net/2445/195344Motivation: Planarians are emerging as a model organism to study regeneration in animals. However, the little available data of protein-protein interactions hinders the advances in understanding the mechanisms underlying its regenerating capabilities. Results: We have developed a protocol to predict protein-protein interactions using sequence homology data and a reference Human interactome. This methodology was applied on eleven Schmidtea mediterranea transcriptomic sequence datasets. Then, using Neo4j as our database manager, we developed PlanNET, a web application to explore the multiplicity of networks and the associated sequence annotations. By mapping RNA-seq expression experiments onto the predicted networks, and allowing a transcript-centric exploration of the planarian interactome, we provide researchers with a useful tool to analyse possible pathways and to design new experiments, as well as a reproducible methodology to predict, store, and explore protein interaction networks for non-model organisms. Availability: The web application PlanNET is available at https://compgen.bio.ub.edu/PlanNET. The source code used is available at https://compgen.bio.ub.edu/PlanNET/downloads.8 p.application/pdfeng(c) Castillo-Lara, Sergio et al., 2017Planària (Gènere)RNAPlanaria (Genus)RNAinfo:eu-repo/semantics/article6772172023-03-15info:eu-repo/semantics/openAccess