Zboromyrska, YuliyaRico, VerónicaPitart, CristinaFernandez Pittol, Mariana JoséSoriano,ÀlexBosch Mestres, Jordi2023-05-122023-05-122022-04-262079-6382https://hdl.handle.net/2445/197926The direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment.Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selected with flow cytometry, with direct identification by MALDI-TOF mass spectrometry, and rapid detection of extended-spectrum beta-lactamase (ESBL) and carbapenemases-producing strains by lateral flow were analyzed.The positive predictive value of the direct identification of the 86 samples that met the flow cytometry criterion (>5000 bacteria/µL) was 96.4%. Reliable direct identification was obtained in 14 of the 27 (51.8%) urinary source bacteraemias. There was 100% agreement between the lateral flow and antibiogram in the detection of ESBL and carbapenemases.the protocol for the direct identification and rapid detection of ESBL and carbapenemases-producing strains from urine samples is a reliable and useful tool.6 p.application/pdfengcc by (c) Zboromyrska, Yuliya et al, 2022http://creativecommons.org/licenses/by/3.0/es/Infeccions del tracte urinariBacteris patògensUrinary tract infectionsPathogenic bacteriaImplementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosisinfo:eu-repo/semantics/article2023-05-02info:eu-repo/semantics/openAccess931528035625226