Benavente Moreno, Fernando J. (Julián)Peró Gascón, RogerPont Villanueva, LauraJaumot Soler, JoaquimBarbosa Torralbo, JoséSanz Nebot, María Victoria2020-02-272020-12-3120180021-9673https://hdl.handle.net/2445/151389We present capillary electrophoresis-mass spectrometry (CE-MS) in combination with advanced chemometric tools for the analysis of bioactive compounds in food, in particular for the identification of antihypertensive peptides in a nutraceutical derived from a bovine milk protein hydrolysate. Different extracts of the nutraceutical were analyzed by CE-MS, and the electropherograms were processed using a novel data analysis workflow that included regions of interest (ROIs) compression and multivariate curve resolution alternating least squares (MCR-ALS). MCR-ALS permitted the description of the nutraceutical extract as ten characteristic components with their electrophoretic profiles and mass spectra. Twenty-two compounds were tentatively identified as antihypertensive bovine casein fragments through a mass search in a database of bioactive peptides. The identity of 17 antihypertensive peptides was reliably confirmed by capillary electrophoresis-tandem mass spectrometry. The proposed analytical approach demonstrated the potential to obtain a characteristic and activity-related fingerprint for quality control and authentication of the antihypertensive nutraceutical.9 p.application/pdfengcc-by-nc-nd (c) Elsevier B.V., 2018http://creativecommons.org/licenses/by-nc-nd/3.0/esPèptidsElectroforesi capil·larQuimiometriaEspectrometria de massesPeptidesCapillary electrophoresisChemometricsMass spectrometryinfo:eu-repo/semantics/article6768012020-02-27info:eu-repo/semantics/openAccess