Benabou Zdaou, SanaeEritja i Casadellà, RamonGargallo Gómez, Raimundo2020-02-262020-02-2620132090-7729https://hdl.handle.net/2445/151262The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature Size Exclusion Chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplex. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles.7 p.application/pdfengcc-by (c) Benabou Zdaou, Sanae et al., 2013http://creativecommons.org/licenses/by/3.0/esÀcids nucleicsG-estructuresOligonucleòtidsNucleic acidsG-structuresOligonucleotidesinfo:eu-repo/semantics/article6284442020-02-26info:eu-repo/semantics/openAccess25937962