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Title: | Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis |
Author: | Gomes, Cláudia Palma, Noemí Pons, Maria J. Magallón Tejada, Ariel Sandoval, Isabel Tinco-Valdez, Carmen Gutarra, Carlos Valle Mendoza, Juana del Ruiz, Joaquim Matsuoka, Mayumi |
Keywords: | Malalties bacterianes Paisatge rural Antígens bacterians Bacterial diseases Rural landscape Bacterial antigens |
Issue Date: | 14-Sep-2016 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | BACKGROUND: Bartonella bacilliformis is the causative agent of Carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. The lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. This study aimed to identify new B. bacilliformis antigenic candidates that could lead to a new diagnostic tool able to be implemented in endemic rural areas. METHODOLOGY/PRINCIPAL FINDINGS: Blood (n = 198) and serum (n = 177) samples were collected in northern Peru. Clinical data were recorded. Specific 16S rRNA amplification by RT-PCR, IFA and ELISA for IgM/IgG with whole cells as antigens was done. Western blot analysis and N-terminal amino acid sequencing detected seroreactive proteins. ELISAs for IgM/IgG for the antigenic candidates were performed. Of the population 33.3% reported at least one symptom compatible with Carrion's disease; 25.4% (IFA), 27.1% (ELISA-IgG), 33.9% (ELISA-IgM) and 38.9% (RT-PCR) of samples were positive. Four proteins were considered potential antigenic candidates, including two new antigenic candidates, succinyl-CoA synthetase subunit alpha (SCS-alpha) and succinyl-CoA synthetase subunit beta (SCS-beta). On Western blot both Pap31 and SCS-alpha interacted with IgM, while GroEL and SCS-beta interacted with IgG. The presence of specific antibodies against the antigenic candidates varied from 34.5% (IgG against SCS-alpha) to 97.2% (IgM against Pap31). CONCLUSIONS/SIGNIFICANCE: RT-PCR and the high levels of positivity for specific ELISAs demonstrate high levels of B. bacilliformis exposure and asymptomatic carriers among inhabitants. The new antigens identified might be used as a new rapid diagnostic tool to diagnose acute Carrion's disease and identify asymptomatic carriers. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pntd.0004989 |
It is part of: | PLoS Neglected Tropical Diseases, 2016, vol. 10, num. 9, p. e0004989 |
URI: | http://hdl.handle.net/2445/102466 |
Related resource: | http://dx.doi.org/10.1371/journal.pntd.0004989 |
ISSN: | 1935-2727 |
Appears in Collections: | Articles publicats en revistes (ISGlobal) |
Files in This Item:
File | Description | Size | Format | |
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gomes2016_2244.pdf | 1.38 MB | Adobe PDF | View/Open |
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