Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/112096
Title: Altered circadian rhythm and metabolic gene profile in rats subjected to advanced light phase shifts
Author: Herrero Rodríguez, Laura
Valcarcel-Jimenez, Lorea
Crhistiane Andressa da Silva
Díez Noguera, Antoni
Cambras Riu, Trinitat
Serra i Cucurull, Dolors
Issue Date: 2-Apr-2015
Publisher: Public Library of Science (PLoS)
Abstract: The circadian clock regulates metabolic homeostasis and its disruption predisposes to obesity and other metabolic diseases. However, the effect of phase shifts on metabolism is not completely understood. We examined whether alterations in the circadian rhythm caused by phase shifts induce metabolic changes in crucial genes that would predispose to obesity. Three-month-old rats were maintained on a standard diet under lighting conditions with chronic phase shifts consisting of advances, delays or advances plus delays. Serum leptin, insulin and glucose levels decreased only in rats subjected to advances. The expression of the clock gene Bmal 1 increased in the hypothalamus, white adipose tissue (WAT), brown adipose tissue (BAT) and liver of the advanced group compared to control rats. The advanced group showed an increase in hypothalamic AgRP and NPY mRNA, and their lipid metabolism gene profile was altered in liver, WAT and BAT. WAT showed an increase in inflammation and ER stress and brown adipocytes suffered a brown-to-white transformation and decreased UCP-1 expression. Our results indicate that chronic phase advances lead to significant changes in neuropeptides, lipid metabolism, inflammation and ER stress gene profile in metabolically relevant tissues such as the hypothalamus, liver, WAT and BAT. This highlights a link between alteration of the circadian rhythm and metabolism at the transcriptional level.
Note: Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0122570
It is part of: PLoS One, 2015, vol. 10, num. 4, p. e0122570
Related resource: https://doi.org/10.1371/journal.pone.0122570
URI: http://hdl.handle.net/2445/112096
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Bioquímica i Fisiologia)

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