Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/112143
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Calderón Domínguez, María | - |
dc.contributor.author | Alcalá, Martín | - |
dc.contributor.author | Sebastián Muñoz, David | - |
dc.contributor.author | Zorzano Olarte, Antonio | - |
dc.contributor.author | Viana Arribas, Marta | - |
dc.contributor.author | Serra i Cucurull, Dolors | - |
dc.contributor.author | Herrero Rodríguez, Laura | - |
dc.date.accessioned | 2017-06-09T07:38:56Z | - |
dc.date.available | 2017-06-09T07:38:56Z | - |
dc.date.issued | 2017-03-13 | - |
dc.identifier.issn | 2198-3844 | - |
dc.identifier.uri | http://hdl.handle.net/2445/112143 | - |
dc.description.abstract | The rediscovery of brown adipose tissue (BAT) in humans and its capacity to oxidize fat and dissipate energy as heat has put the spotlight on its potential as a therapeutic target in the treatment of several metabolic conditions including obesity and diabetes. To date the measurement of bioenergetics parameters has required the use of cultured cells or extracted mitochondria with the corresponding loss of information in the tissue context. Herein, we present a method to quantify mitochondrial bioenergetics directly in BAT. Based on XF Seahorse Technology, we assessed the appropriate weight of the explants, the exact concentration of each inhibitor in the reaction, and the specific incubation time to optimize bioenergetics measurements. Our results show that BAT basal oxygen consumption is mostly due to proton leak. In addition, BAT presents higher basal oxygen consumption than white adipose tissue and a positive response to b-adrenergic stimulation. Considering the whole tissue and not just subcellular populations is a direct approach that provides a realistic view of physiological respiration. In addition, it can be adapted to analyze the effect of potential activators of thermogenesis, or to assess the use of fatty acids or glucose as a source of energy. | - |
dc.format.extent | 5 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Wiley-VCH Verlag | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1002/advs.201600274 | - |
dc.relation.ispartof | Advanced Science, 2017, vol. 4, num. 4, p. 1620074 | - |
dc.relation.uri | https://doi.org/10.1002/advs.201600274 | - |
dc.rights | cc-by (c) Calderón et al., 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Bioquímica i Fisiologia) | - |
dc.subject.classification | Teixit adipós | - |
dc.subject.classification | Bioenergètica | - |
dc.subject.classification | Mitocondris | - |
dc.subject.other | Adipose tissues | - |
dc.subject.other | Bioenergetics | - |
dc.subject.other | Mitochondria | - |
dc.title | Brown adipose tissue bioenergetics: a new methodological approach | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2017-06-09T07:38:56Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 28435771 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Articles publicats en revistes (Institut de Biomedicina (IBUB)) Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
671263.pdf | 588.56 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License