Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/112377
Title: A Novel chip for cyclic stretch and intermittent hypoxia cell exposures mimicking obstructive sleep apnea
Author: Campillo, Noelia
Jorba, Ignasi
Schaedel, Laura
Casals, Blai
Gozal, David
Farré Ventura, Ramon
Almendros López, Isaac
Navajas Navarro, Daniel
Keywords: Síndromes d'apnea del son
Oxigen en l'organisme
Respiració
Sleep apnea syndromes
Oxygen in the body
Respiration
Issue Date: 29-Jul-2016
Publisher: Frontiers Media
Abstract: Intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), plays a critical role in the pathogenesis of OSA-associated morbidities, especially in the cardiovascular and respiratory systems. Oxidative stress and inflammation induced by IH are suggested as main contributors of end-organ dysfunction in OSA patients and animal models. Since the molecular mechanisms underlying these in vivo pathological responses remain poorly understood, implementation of experimental in vitro cell-based systems capable of inducing high-frequency IH would be highly desirable. Here, we describe the design, fabrication, and validation of a versatile chip for subjecting cultured cells to fast changes in gas partial pressure and to cyclic stretch. The chip is fabricated with polydimethylsiloxane (PDMS) and consists of a cylindrical well-covered by a thin membrane. Cells cultured on top of the membrane can be subjected to fast changes in oxygen concentration (equilibrium time similar to 6 s). Moreover, cells can be subjected to cyclic stretch at cardiac or respiratory frequencies independently or simultaneously. Rat bone marrow-derived mesenchymal stem cells (MSCs) exposed to IH mimicking OSA and cyclic stretch at cardiac frequencies revealed that hypoxia-inducible factor 1 alpha (HIF-1 alpha) expression was increased in response to both stimuli. Thus, the chip provides a versatile tool for the study of cellular responses to cyclical hypoxia and stretch.
Note: Reproducció del document publicat a: https://doi.org/10.3339/fphys.2016.00319
It is part of: Frontiers in Physiology, 2016, vol. 7, num. 319
Related resource: https://doi.org/10.3339/fphys.2016.00319
URI: http://hdl.handle.net/2445/112377
ISSN: 1664-042X
Appears in Collections:Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

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