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Title: The incC sequence is required for R27 plasmid stability
Author: Tassinari, Eleonora
Aznar, Sonia
Urcola, Imanol
Prieto, Alejandro
Hüttener Queiroz, Mário
Juárez Giménez, Antonio
Keywords: Seqüència d'aminoàcids
Amino acid sequence
Issue Date: 3-May-2016
Publisher: Frontiers Media
Abstract: IncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show similarity to any known ORFs. We performed a deletion analysis of all non-essential R27 DNA sequences to search for hitherto non-identified plasmid functions that might be required for plasmid stability. We report the identification of a short DNA sequence (incC) that is essential for R27 stability. That region contains several repeats (incC repeats), belongs to one of the three-plasmid replicons (R27 FIA-like) and is targeted by the R27 E protein. Deletion of the incC sequence drastically reduces R27 stability both in Escherichia coli and in Salmonella, the effect being more pronounced in this latter species. Interfering with incC-E protein interaction must lead to a reduced IncHI1 plasmid stability, and may represent a new approach to combat antimicrobial resistance.
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It is part of: Frontiers in Microbiology, 2016, vol. 7, num. 6, p. 629
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ISSN: 1664-302X
Appears in Collections:Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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