Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/121071
Title: Synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer containing biotin for selective protein nanopatterning
Author: Lagunas, Anna
Sasso, B.
Tesson, N.
Cantos, C.
Martínez, Elena
Samitier i Martí, Josep
Keywords: Copolímers
Nanoestructures
Nanotecnologia
Copolymers
Nanostructures
Nanotechnology
Issue Date: 22-Oct-2016
Publisher: Royal Society of Chemistry
Abstract: Protein patterning is of interest in high-throughput screening. Due to an increase in demand for further miniaturization of protein assays, block copolymers (BCPs) that can undergo large-area phase separation into nanometer-size domains have attracted great attention as substrates for protein nanopatterning. Here we report the synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer which, once spin-coated, is capable of self-segregating into cylindrical polystyrene (PS) domains. In this copolymer, the PS block was modified to introduce biotin below 10% molar in order to achieve molecular recognition of streptavidin. The PMMA matrix used to introduce poly(ethylene glycol) enabled us to obtain an antifouling environment that prevents unspecific protein adsorption outside the domains. The use of the biotin-streptavidin pair in this BCP makes it suitable for nanopatterning of other biotinylated proteins of interest for the purposes of cell biology, biosensors, and tissue engineering.
Note: Versió postprint del document publicat a: https://doi.org/10.1039/C5PY01601K
It is part of: Polymer Chemistry, 2016, vol. 7, num. 1
URI: http://hdl.handle.net/2445/121071
Related resource: https://doi.org/10.1039/C5PY01601K
ISSN: 1759-9954
Appears in Collections:Articles publicats en revistes (Electrònica)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Publicacions de projectes de recerca finançats per la UE

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