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Title: Nanopatterns of surface-bound ephrinB1 produce multivalent ligand-receptor interactions that tune EphB2 receptor clustering
Author: Hortigüela, Verónica
Larrañaga, Enara
Cutrale, Francesco
Seriola, Anna
García Díaz, María
Lagunas, Anna
Andilla, Jordi
Loza Alvarez, Pablo
Samitier i Martí, Josep
Ojosnegros, Sanuel
Martínez, Elena
Keywords: Copolímers
Materials nanoestructurats
Nanostructured materials
Issue Date: 15-Dec-2017
Publisher: American Chemical Society
Abstract: Here we present a nanostructured surface able to produce multivalent interactions between surface-bound ephrinB1 ligands and membrane EphB2 receptors. We created ephrinB1 nanopatterns of regular size (<30 nm in diameter) by using self-assembled diblock copolymers. Next, we used a statistically enhanced version of the Number and Brightness technique, which can discriminate with molecular sensitivity the oligomeric states of diffusive species to quantitatively track the EphB2 receptor oligomerization process in real time. The results indicate that a stimulation using randomly distributed surface-bound ligands was not sufficient to fully induce receptor aggregation. Conversely, when nanopatterned onto our substrates, the ligands effectively induced a strong receptor oligomerization. This presentation of ligands improved the clustering efficiency of conventional ligand delivery systems, as it required a 9-fold lower ligand surface coverage and included faster receptor clustering kinetics compared to traditional cross-linked ligands. In conclusion, nanostructured diblock copolymers constitute a novel strategy to induce multivalent ligand-receptor interactions leading to a stronger, faster, and more efficient receptor activation, thus providing a useful strategy to precisely tune and potentiate receptor responses. The efficiency of these materials at inducing cell responses can benefit applications such as the design of new bioactive materials and drug-delivery systems.
Note: Versió postprint del document publicat a:
It is part of: Nano Letters, 2017, vol. 18, num. 1, p. 629-637
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ISSN: 1530-6984
Appears in Collections:Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Electrònica)
Publicacions de projectes de recerca finançats per la UE

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