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Title: Evidence for functional pre-coupled complexes of receptor heteromers and adenylyl cyclase
Author: Navarro Brugal, Gemma
Cordomí, Arnau
Casadó Anguera, Verònica
Moreno Guillén, Estefanía
Cai, Ning-Sheng
Cortes, Antoni
Canela Campos, Enric I.
Dessauer, Carmen W.
Casadó, Vicent
Pardo, Leonardo
Lluís i Biset, Carme
Ferré, Sergi
Keywords: Adenosina
Receptors cel·lulars
Cell receptors
Issue Date: 28-Mar-2018
Publisher: Nature Publishing Group
Abstract: G protein-coupled receptors (GPCRs), G proteins and adenylyl cyclase (AC) comprise one of the most studied transmembrane cell signaling pathways. However, it is unknown whether the ligand-dependent interactions between these signaling molecules are based on random collisions or the rearrangement of pre-coupled elements in a macromolecular complex. Furthermore, it remains controversial whether a GPCR homodimer coupled to a single heterotrimeric G protein constitutes a common functional unit. Using a peptide-based approach, we here report evidence for the existence of functional pre-coupled complexes of heteromers of adenosine A2A receptor and dopamine D2 receptor homodimers coupled to their cognate Gs and Gi proteins and to subtype 5 AC. We also demonstrate that this macromolecular complex provides the necessary frame for the canonical Gs-Gi interactions at the AC level, sustaining the ability of a Gi-coupled GPCR to counteract AC activation mediated by a Gs-coupled GPCR.
Note: Reproducció del document publicat a:
It is part of: Nature Communications, 2018, vol. 9, num. 1, pp.1242
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ISSN: 2041-1723
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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